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However, external stimuli, like UV radiation, cause phosphorylation and degradation of IκB, allowing NF-κB p65 to enter the nucleus and regulate target genes, including the Bcl-2 family and pro-oxidative agents, iNOS [157,158,159]

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After washing, the membranes were incubated with corresponding HRP-conjugated secondary antibodies (1:5,000) at 25°C for 1.5 h