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Immunohistochemical detection of p53 and progerin, were performed on paraffin sections (3 μm), and subsequent sections were exposed to horseradish peroxidase (HRP)-antibody colored with DAB, and visualized by microscopy (BX51, Olympus, Japan)

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They mitigate the oxidative damage to membrane phospholipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), and restore ceramide (CER) synthesis, which promotes apoptotic pathways in melanoma cells without harming healthy melanocytes 162