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Protein preparation Escherichia coli glnH was cloned from genomic DNA of Escherichia coli BL21(DE3) strain by standard PCR and subcloned into a pET28a vector between Eco R I and Sal I

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They also showed that oral administration of green tea extract reduced inflammatory cell infiltration and the patchy fibrosis in pulmonary fibrosis and inflammation mouse model induced by intratracheal instillation of fluorescein isothiocyanate (FITC) (Donà et al., 2003)