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Carbamidomethylation was set as a fixed modification, whereas methionine oxidation, asparagine and glutamine deamination, and glycosylation options were set as variable modifications, according to published data ( The intact hCG analysis revealed several hCGα glycoforms

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10MG:79

However, the key difference between the two HPLC methods was the use of different columns and mobile phases [38,39]